superresolution structured illumination microscope  (Nikon)

Journal: Molecular Biology of the Cell

Article Title: Neuroprotective astrocyte-derived insulin/insulin-like growth factor 1 stimulates endocytic processing and extracellular release of neuron-bound Aβ oligomers

doi: 10.1091/mbc.E17-06-0416

Figure Lengend Snippet: SIM enables precise imaging of AβOs bound to spines. SIM was used to determine the binding of AβOs to primary hippocampal neurons, using the N-SIM superresolution microscope with a lateral resolution at 100× of ∼100 nm, compared with the more typical 400 nm of most microscopes. Primary hippocampal cells, cultured for 19 d, were treated with 100 nM AβOs for 1 h and immunolabeled with anti-TuJ (green) and NU4 (magenta).

Article Snippet: N-SIM images were captured on a Nikon N-SIM Structured Illumination superresolution microscope.

Techniques: Imaging, Binding Assay, Microscopy, Cell Culture, Immunolabeling